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KMID : 0545120090190121695
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 12 p.1695 ~ p.1702
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A high-yielding, generic fed-batch process for recombinant antibody production of GS-engineered cell lines
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Fan Li
Liang Zhao
Yating Sun
Tianci Kou
Yan Zhou
Tan Wen-Song
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Abstract
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An animal component-free and chemically defined fed-batch process for GS-engineered cell lines producing recombinant antibodies has been developed. The fed-batch process relied on supplying sufficient nutrients to match their consumption, simultaneously minimizing the accumulation of byproducts (lactate and osmolality). The proportionalities of nutritional consumption were determined by direct analysis. And the robust, metabolically responsive feeding strategy was based on the off-line measurement of glucose. The fed-batch process was shown to perform equivalently in GS-CHO and GS-NS0 culture. Compared to batch cultures, the fed-batch technology generated the magnitude of the increase in cell yields (5 fold) and final antibody concentrations (4-8 fold). The majority of the increase in final antibody concentration was functions of the increased cell density and the prolonged culture time. This generic and high-yielding fed-batch process would shorten development time, and ensure process stability, thereby facilitating the manufacture of therapeutic antibodies by GS-engineered cell lines.
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KEYWORD
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high-yielding, generic fed-batch process, GS-engineered cell lines, glucose, recombinant antibody
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